Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response.

ABSTRACT:

Objective

Hyposalivation-related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head and neck cancer. Stem cell therapy is an attractive option of treatment, but demands knowledge of stem cell functioning. Therefore, we aimed to develop a murine parotid gland organoid model to explore radiation response of stem cells in vitro.

Materials and methods

Single cells derived from murine parotid gland organoids were passaged in Matrigel with defined medium to assess self-renewal and differentiation potential. Single cells were irradiated and plated in a 3D clonogenic stem cell survival assay to assess submandibular and parotid gland radiation response.

Results

Single cells derived from parotid gland organoids were able to extensivel

SUBMITTER: Serrano Martinez P 

PROVIDER: S-EPMC7818507 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature