Slit/Robo signaling regulates Leydig cell steroidogenesis.

ABSTRACT:

Background

First identified as a regulator of neuronal axon guidance, Slit/Robo signaling has since been implicated in additional physiologic and pathologic processes, such as angiogenesis, organogenesis and cancer progression. However, its roles in the regulation of testis function have been little explored.

Methods

Immunohistochemistry and RT-qPCR analyses were performed to detect the expression of Slit/Robo signaling effectors in the adult mouse testis. To identify the roles and mechanisms of Slit/Robo signaling in the regulation of steroidogenesis, RT-qPCR, immunoblotting and hormone measurements were carried out using Leydig cells (primary cultures and the MA10 cell line) treated with exogenous SLIT ligands, and testes from Robo1-null mice.

Results

Slit1, -2 and

SUBMITTER: Martinot E 

PROVIDER: S-EPMC7819258 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature