Evaluation of holographic imaging cytometer holomonitor M4® motility applications.

ABSTRACT: Digital holographic cytometry (DHC) and other methods of quantitative phase imaging permit extended time-lapse imaging of mammalian cells in the absence of induced cellular toxicity. Manufactured DHC platforms equipped with semi-automated image acquisition, segmentation, and analysis software packages (or modules) for assessing cell behavior are now commercially available. When housed in mammalian cell incubators these cytometers offer the potential to monitor and quantify a range of cellular behaviors without disrupting routine culture. Realization of this potential requires validation against established standards. Two proprietary software modules for assessing cellular motility available using the HoloMonitor M4 DHC platform were evaluated on human melanoma cells lines with known relati

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7819361 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature