Optimization of CRISPR/Cas System for Improving Genome Editing Efficiency in Plasmodium falciparum.

ABSTRACT: Studies of molecular mechanisms and related gene functions have long been restricted by limited genome editing technologies in malaria parasites. Recently, a simple and effective genome editing technology, the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system, has greatly facilitated these studies in many organisms, including malaria parasites. However, due to the special genome feature of malaria parasites, the manipulation and gene editing efficacy of the CRISPR/Cas system in this pathogen need to be improved, particularly in the human malaria parasite, Plasmodium falciparum. Herein, based on the CRISPR/Cas9 system, we developed an integrating strategy to generate a Cas9i system, which significantly shortened the time for generation of

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7819880 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature