Rice protein-binding microarrays: a tool to detect cis-acting elements near promoter regions in rice.

ABSTRACT:

Main conclusion

The present study showed that a rice (Oryza sativa)-specific protein-binding microarray (RPBM) can be applied to analyze DNA-binding motifs with a TF where binding is evaluated in extended natural promoter regions. The analysis may facilitate identifying TFs and their downstream genes and constructing gene networks through cis-elements. Transcription factors (TFs) regulate gene expression at the transcriptional level by binding a specific DNA sequence. Thus, predicting the DNA-binding motifs of TFs is one of the most important areas in the functional analysis of TFs in the postgenomic era. Although many methods have been developed to address this challenge, many TFs still have unknown DNA-binding motifs. In this study, we designed RPBM with 40-bp probes and 20-bp of

SUBMITTER: Kim JS 

PROVIDER: S-EPMC7819943 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature