A Novel Dual Antibody Staining Assay to Measure Estrogen Receptor Transcriptional Activity.

ABSTRACT: Activity of the canonical estrogen receptor (ER) pathway is equivalent to functional activity of the nuclear ER transcription factor. Monoclonal antibodies (MoAbs) that identify nuclear ER in cells and tissue samples are frequently used to assess ER transcriptional activity, however, it remains unclear if this approach is sufficiently predictive of ER pathway activity. This study uses ER-positive breast cancer cell lines (MCF7 and T47D) in which ER transcriptional activity was quantified using an mRNA-based ER pathway activity assay. The relationship between ER activity and nuclear ER staining with ER MoAbs was then investigated. Confirming earlier findings, the results show that while the presence of ER in the cell nucleus is a prerequisite for ER activity, it is not predictive of ER tran

SUBMITTER: van Hemert F 

PROVIDER: S-EPMC7820081 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature