Rigorous Plasma Microbiome Analysis Method Enables Disease Association Discovery in Clinic.

ABSTRACT: Blood microbiome is important to investigate microbial-host interactions and the effects on systemic immune perturbations. However, this effort has met with major challenges due to low microbial biomass and background artifacts. In the current study, microbial 16S DNA sequencing was applied to analyze plasma microbiome. We have developed a quality-filtering strategy to evaluate and exclude low levels of microbial sequences, potential contaminations, and artifacts from plasma microbial 16S DNA sequencing analyses. Furthermore, we have applied our technique in three cohorts, including tobacco-smokers, HIV-infected individuals, and individuals with systemic lupus erythematosus (SLE), as well as corresponding controls. More than 97% of total sequence data was removed using stringent quality-fi

SUBMITTER: Luo Z 

PROVIDER: S-EPMC7820181 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature