Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT.

ABSTRACT: Epithelial to mesenchymal transition (EMT) is a critical cellular process that has been well characterized during embryonic development and cancer metastasis and it also is implicated in several physiological and pathological events including embryonic stem cell differentiation. During early stages of differentiation, human embryonic stem cells pass through EMT where deeper morphological, molecular and biochemical changes occur. Though initially considered as a decision between two states, EMT process is now regarded as a fluid transition where cells exist on a spectrum of intermediate states. In this work, using a CRISPR interference system in human embryonic stem cells, we describe a molecular characterization of the effects of downregulation of E-cadherin, one of the main initiation eve

SUBMITTER: Aban CE 

PROVIDER: S-EPMC7820496 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature