Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography.

ABSTRACT: Quantification of anatomical and compositional features underpins both fundamental and applied studies of plant structure and function. Relatively few non-invasive techniques are available for aquatic plants. Traditional methods such as sectioning are low-throughput and provide 2-dimensional information. X-ray Computed Microtomography (μCT) offers a non-destructive method of three dimensional (3D) imaging in planta, but has not been widely used for aquatic species, due to the difficulties in sample preparation and handling. We present a novel sample handling protocol for aquatic plant material developed for μCT imaging, using duckweed plants and turions as exemplars, and compare the method against existing approaches. This technique allows for previously unseen 3D volume analysis of

SUBMITTER: Jones DH 

PROVIDER: S-EPMC7820725 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature