Light sheet fluorescence microscopy as a new method for unbiased three-dimensional analysis of vascular injury.

ABSTRACT:

Aims

Assessment of preclinical models of vascular disease is paramount in the successful translation of novel treatments. The results of these models have traditionally relied on two-dimensional (2D) histological methodologies. Light sheet fluorescence microscopy (LSFM) is an imaging platform that allows for three-dimensional (3D) visualization of whole organs and tissues. In this study, we describe an improved methodological approach utilizing LSFM for imaging of preclinical vascular injury models while minimizing analysis bias.

Methods and results

The rat carotid artery segmental pressure-controlled balloon injury and mouse carotid artery ligation injury were performed. Arteries were harvested and processed for LSFM imaging and 3D analysis, as well as for 2D area histologi

SUBMITTER: Buglak NE 

PROVIDER: S-EPMC7820842 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature