Facile Stereoselective Reduction of Prochiral Ketones by using an F₄₂₀ -dependent Alcohol Dehydrogenase.

ABSTRACT: Effective procedures for the synthesis of optically pure alcohols are highly valuable. A commonly employed method involves the biocatalytic reduction of prochiral ketones. This is typically achieved by using nicotinamide cofactor-dependent reductases. In this work, we demonstrate that a rather unexplored class of enzymes can also be used for this. We used an F₄₂₀ -dependent alcohol dehydrogenase (ADF) from Methanoculleus thermophilicus that was found to reduce various ketones to enantiopure alcohols. The respective (S) alcohols were obtained in excellent enantiopurity (>99 % ee). Furthermore, we discovered that the deazaflavoenzyme can be used as a self-sufficient system by merely using a sacrificial cosubstrate (isopropanol) and a catalytic amount of cofactor F₄₂₀ or

SUBMITTER: Martin C 

PROVIDER: S-EPMC7820951 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature