Molecular characterization of Cryptosporidium isolates from humans in Ontario, Canada.

ABSTRACT:

Background

Cryptosporidiosis is a gastrointestinal disease with global distribution. It has been a reportable disease in Canada since 2000; however, routine molecular surveillance is not conducted. Therefore, sources of contamination are unknown. The aim of this project was to identify species and subtypes of Cryptosporidium in clinical cases from Ontario, the largest province in Canada, representing one third of the Canadian population, in order to understand transmission patterns.

Methods

A total of 169 frozen, banked, unpreserved stool specimens that were microscopy positive for Cryptosporidium over the period 2008-2017 were characterized using molecular tools. A subset of the 169 specimens were replicate samples from individual cases. DNA was extracted directly from the

SUBMITTER: Guy RA 

PROVIDER: S-EPMC7821412 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature