MicroExonator enables systematic discovery and quantification of microexons across mouse embryonic development.

ABSTRACT:

Background

Microexons, exons that are ≤ 30 nucleotides, are a highly conserved and dynamically regulated set of cassette exons. They have key roles in nervous system development and function, as evidenced by recent results demonstrating the impact of microexons on behaviour and cognition. However, microexons are often overlooked due to the difficulty of detecting them using standard RNA-seq aligners.

Results

Here, we present MicroExonator, a novel pipeline for reproducible de novo discovery and quantification of microexons. We process 289 RNA-seq datasets from eighteen mouse tissues corresponding to nine embryonic and postnatal stages, providing the most comprehensive survey of microexons available for mice. We detect 2984 microexons, 332 of which are differentially spliced

SUBMITTER: Parada GE 

PROVIDER: S-EPMC7821500 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature