Detection of bluetongue virus in Culicoides spp. in southern Yunnan Province, China.

ABSTRACT:

Background

Culicoides (Diptera: Ceratopogonidae) are vectors for many arboviruses. At least 20 species are considered as vectors or potential vectors of bluetongue virus (BTV) which cause bluetongue disease in ruminants. A BTV prevalence of 30-50% among cattle and goats in tropical southern Yunnan Province, China, prompted an investigation of the potential BTV vectors in this area.

Methods

Culicoides were collected by light trapping at three sites in the tropical region of Yunnan Province. Species were identified based on morphology and DNA sequences of cytochrome c oxidase subunit 1 (cox1). PCR and quantitative PCR following reverse transcription were used to test for the presence of BTV RNA in these specimens. Phylogenetic analysis was used to analyze the cox1 sequences of

SUBMITTER: Duan YL 

PROVIDER: S-EPMC7821528 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature