Regulatory encoding of quantitative variation in spatial activity of a Drosophila enhancer.

ABSTRACT: Developmental enhancers control the expression of genes prefiguring morphological patterns. The activity of an enhancer varies among cells of a tissue, but collectively, expression levels in individual cells constitute a spatial pattern of gene expression. How the spatial and quantitative regulatory information is encoded in an enhancer sequence is elusive. To link spatial pattern and activity levels of an enhancer, we used systematic mutations of the yellow spot enhancer, active in developing Drosophila wings, and tested their effect in a reporter assay. Moreover, we developed an analytic framework based on the comprehensive quantification of spatial reporter activity. We show that the quantitative enhancer activity results from densely packed regulatory information along the sequence, an

SUBMITTER: Le Poul Y 

PROVIDER: S-EPMC7821883 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature