Comparative Analysis of Carbohydrate Active Enzymes in the Flammulina velutipes var. lupinicola Genome.

ABSTRACT: The purpose of this study was to determine the genome sequence of Flammulina velutipes var. lupinicola based on next-generation sequencing (NGS) and to identify the genes encoding carbohydrate-active enzymes (CAZymes) in the genome. The optimal assembly (71 kmer) based on ABySS de novo assembly revealed a total length of 33,223,357 bp (49.53% GC content). A total of 15,337 gene structures were identified in the F. velutipes var. lupinicola genome using ab initio gene prediction method with Funannotate pipeline. Analysis of the orthologs revealed that 11,966 (96.6%) out of the 15,337 predicted genes belonged to the orthogroups and 170 genes were specific for F. velutipes var. lupinicola. CAZymes are divided into six classes: auxiliary activit

SUBMITTER: Yu HW 

PROVIDER: S-EPMC7822412 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature