Interferon Regulatory Factor-5 in Resident Macrophage Promotes Polycystic Kidney Disease.

ABSTRACT:

Background

Autosomal dominant polycystic kidney disease is caused by genetic mutations in PKD1 or PKD2. Macrophages and their associated inflammatory cytokines promote cyst progression; however, transcription factors within macrophages that control cytokine production and cystic disease are unknown.

Methods

In these studies, we used conditional Pkd1 mice to test the hypothesis that macrophage-localized interferon regulatory factor-5 (IRF5), a transcription factor associated with production of cyst-promoting cytokines (TNFα, IL-6), is required for accelerated cyst progression in a unilateral nephrectomy (1K) model. Analyses of quantitative real-time PCR (qRT-PCR) and flow-cytometry data 3 weeks post nephrectomy, a time point before the onset of sev

SUBMITTER: Zimmerman KA 

PROVIDER: S-EPMC7822573 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature