Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures.

ABSTRACT: Although conformational dynamics of RNA molecules are potentially important in microRNA (miRNA) processing, the role of the protein binding partners in facilitating the requisite structural changes is not well understood. In previous work, we and others have demonstrated that nonduplex structural elements and the conformational flexibility they support are necessary for efficient RNA binding and cleavage by the proteins associated with the two major stages of miRNA processing. However, recent studies showed that the protein DGCR8 binds primary miRNA and duplex RNA with similar affinities. Here, we study RNA binding by a small recombinant construct of the DGCR8 protein and the RNA conformation changes that result. This construct, the DGCR8 core, contains two double-stranded RNA-binding doma

SUBMITTER: Pabit SA 

PROVIDER: S-EPMC7822732 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature