Cell Size Is Coordinated with Cell Cycle by Regulating Initiator Protein DnaA in E. coli.

ABSTRACT: Sixty years ago, bacterial cell size was found to be an exponential function of growth rate. Fifty years ago, a more general relationship was proposed, in which cell mass was equal to the initiation mass multiplied by 2 to the power of the ratio of the total time of C and D periods to the doubling time. This relationship has recently been experimentally confirmed by perturbing doubling time, C period, D period, or initiation mass. However, the underlying molecular mechanism remains unclear. Here, we developed a theoretical model for initiator protein DnaA mediating DNA replication initiation in Escherichia coli. We introduced an initiation probability function for competitive binding of DnaA-ATP and DnaA-ADP at oriC. We established a kinetic description of regulatory processes (e.g., expre

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC7822741 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature