Direct Mapping from Intracellular Chemotaxis Signaling to Single-Cell Swimming Behavior.

ABSTRACT: Bacterial chemotaxis allows bacteria to sense the chemical environment and modulate their swimming behavior accordingly. Although the intracellular chemotaxis signaling pathway has been studied extensively, experimental studies are still lacking that could provide direct link from the pathway output (the intracellular concentration of the phosphorylated form of the response regulator phosphorylated CheY (CheY-P)) to single-cell swimming behavior. Here, we measured the swimming behavior of individual Escherichia coli cells while simultaneously detecting the intracellular CheY-P concentration, thereby providing a direct relationship between the intracellular CheY-P concentration and the single-cell run-and-tumble behavior. The measured relationship is consistent with the ultrasensitivity of

SUBMITTER: Tao A 

PROVIDER: S-EPMC7822746 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature