High-resolution targeted 3C interrogation of cis-regulatory element organization at genome-wide scale.

ABSTRACT: Chromosome conformation capture (3C) provides an adaptable tool for studying diverse biological questions. Current 3C methods generally provide either low-resolution interaction profiles across the entire genome, or high-resolution interaction profiles at limited numbers of loci. Due to technical limitations, generation of reproducible high-resolution interaction profiles has not been achieved at genome-wide scale. Here, to overcome this barrier, we systematically test each step of 3C and report two improvements over current methods. We show that up to 30% of reporter events generated using the popular in situ 3C method arise from ligations between two individual nuclei, but this noise can be almost entirely eliminated by isolating intact nuclei after ligation. Using Nuclear-Titrated Captu

SUBMITTER: Downes DJ 

PROVIDER: S-EPMC7822813 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature