LINE-1 retrotransposon methylation in chorionic villi of first trimester miscarriages with aneuploidy.

ABSTRACT:

Purpose

High frequency of aneuploidy in meiosis and cleavage stage coincides with waves of epigenetic genome reprogramming that may indicate a possible association between epigenetic mechanisms and aneuploidy occurrence. This study aimed to assess the methylation level of the long interspersed repeat element 1 (LINE-1) retrotransposon in chorionic villi of first trimester miscarriages with a normal karyotype and aneuploidy.

Methods

The methylation level was assessed at 19 LINE-1 promoter CpG sites in chorionic villi of 141 miscarriages with trisomy of chromosomes 2, 6, 8-10, 13-15, 16, 18, 20-22, and monosomy X using massive parallel sequencing.

Results

The LINE-1 methylation level was elevated statistically significant in chorionic villi of miscarriages with both tri

SUBMITTER: Vasilyev SA 

PROVIDER: S-EPMC7823001 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature