Differentiating Two Closely Related Alexandrium Species Using Comparative Quantitative Proteomics.

ABSTRACT: Alexandrium minutum and Alexandrium tamutum are two closely related harmful algal bloom (HAB)-causing species with different toxicity. Using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics and two-dimensional differential gel electrophoresis (2D-DIGE), a comprehensive characterization of the proteomes of A. minutum and A. tamutum was performed to identify the cellular and molecular underpinnings for the dissimilarity between these two species. A total of 1436 proteins and 420 protein spots were identified using iTRAQ-based proteomics and 2D-DIGE, respectively. Both methods revealed little difference (10-12%) between the proteomes of A. minutum and A. tamutum, highlighting that these organisms follow simila

SUBMITTER: Subong BJJ 

PROVIDER: S-EPMC7823455 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature