An Electrokinetically-Driven Microchip for Rapid Entrapment and Detection of Nanovesicles.

ABSTRACT: Electrical Impedance Spectroscopy (EIS) has been widely used as a label-free and rapid characterization method for the analysis of cells in clinical research. However, the related work on exosomes (40-150 nm) and the particles of similar size has not yet been reported. In this study, we developed a new Lab-on-a-Chip (LOC) device to rapidly entrap a cluster of sub-micron particles, including polystyrene beads, liposomes, and small extracellular vesicles (exosomes), utilizing an insulator-based dielectrophoresis (iDEP) scheme followed by measuring their impedance utilizing an integrated electrical impedance sensor. This technique provides a label-free, fast, and non-invasive tool for the detection of bionanoparticles based on their unique dielectric properties. In the future, this device cou

SUBMITTER: Shi L 

PROVIDER: S-EPMC7823576 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature