β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization.

ABSTRACT: Stabilization ponds are a common treatment technology for wastewater generated by dairy industries. Large proportions of cheese whey are thrown into these ponds, creating an environmental problem because of the large volume produced and the high biological and chemical oxygen demands. Due to its composition, mainly lactose and proteins, it can be considered as a raw material for value-added products, through physicochemical or enzymatic treatments. β-Galactosidases (EC 3.2.1.23) are lactose modifying enzymes that can transform lactose in free monomers, glucose and galactose, or galactooligosacharides. Here, the identification of novel genes encoding β-galactosidases, identified via whole-genome shotgun sequencing of the metagenome of dairy industries stabilization ponds is reported. The ge

SUBMITTER: Eberhardt MF 

PROVIDER: S-EPMC7823827 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature