N-terminal VP1 Truncations Favor T = 1 Norovirus-Like Particles.

ABSTRACT: Noroviruses cause immense sporadic gastroenteritis outbreaks worldwide. Emerging genotypes, which are divided based on the sequence of the major capsid protein VP1, further enhance this public threat. Self-assembling properties of the human norovirus major capsid protein VP1 are crucial for using virus-like particles (VLPs) for vaccine development. However, there is no vaccine available yet. Here, VLPs from different variants produced in insect cells were characterized in detail using a set of biophysical and structural tools. We used native mass spectrometry, gas-phase electrophoretic mobility molecular analysis, and proteomics to get clear insights into particle size, structure, and composition, as well as stability. Generally, noroviruses have been known to form mainly T = 3 part

SUBMITTER: Pogan R 

PROVIDER: S-EPMC7824077 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature