New Insights into the Ecology and Physiology of Methanomassiliicoccales from Terrestrial and Aquatic Environments.

ABSTRACT: Members of the archaeal order Methanomassiliicoccales are methanogens mainly associated with animal digestive tracts. However, environmental members remain poorly characterized as no representatives not associated with a host have been cultivated so far. In this study, metabarcoding screening combined with quantitative PCR analyses on a collection of diverse non-host-associated environmental samples revealed that Methanomassiliicoccales were very scarce in most terrestrial and aquatic ecosystems. Relative abundance of Methanomassiliicoccales and substrates/products of methanogenesis were monitored during incubation of environmental slurries. A sediment slurry enriched in Methanomassiliicoccales was obtained from a freshwater sample. It allowed the reconstruction

SUBMITTER: Cozannet M 

PROVIDER: S-EPMC7824343 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature