CRISPR/Cas9 Induced Somatic Recombination at the CRTISO Locus in Tomato.

ABSTRACT: Homologous recombination (HR) in somatic cells is not as well understood as meiotic recombination and is thought to be rare. In a previous study, we showed that Inter-Homologous Somatic Recombination (IHSR) can be achieved by targeted induction of DNA double-strand breaks (DSBs). Here, we designed a novel IHSR assay to investigate this phenomenon in greater depth. We utilized F₁ hybrids from divergent parental lines, each with a different mutation at the Carotenoid isomerase (CRTISO) locus. IHSR events, namely crossover or gene conversion (GC), between the two CRTISO mutant alleles (tangerine color) can restore gene activity and be visualized as gain-of-function, wildtype (red) phenotypes. Our results show that out of four intron DSB targets tested, three sh

SUBMITTER: Ben Shlush I 

PROVIDER: S-EPMC7824628 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature