New Insectotoxin from Tibellus Oblongus Spider Venom Presents Novel Adaptation of ICK Fold.

ABSTRACT: The Tibellus oblongus spider is an active predator that does not spin webs and remains poorly investigated in terms of venom composition. Here, we present a new toxin, named Tbo-IT2, predicted by cDNA analysis of venom glands transcriptome. The presence of Tbo-IT2 in the venom was confirmed by proteomic analyses using the LC-MS and MS/MS techniques. The distinctive features of Tbo-IT2 are the low similarity of primary structure with known animal toxins and the unusual motif of 10 cysteine residues distribution. Recombinant Tbo-IT2 (rTbo-IT2), produced in E. coli using the thioredoxin fusion protein strategy, was structurally and functionally studied. rTbo-IT2 showed insecticidal activity on larvae of the housefly Musca domestica (LD₁₀₀ 200 μg/g) and no activ

SUBMITTER: Korolkova Y 

PROVIDER: S-EPMC7824768 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature