A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization.

ABSTRACT: Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema,

SUBMITTER: Teixeira-Cruz JM 

PROVIDER: S-EPMC7824798 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature