MiR-210 and miR-152 as Biomarkers by Liquid Biopsy in Invasive Ductal Carcinoma.

ABSTRACT: Detecting circulating microRNAs (miRNAs; miRs) by means of liquid biopsy is an important tool for the early diagnosis and prognosis of breast cancer (BC). We aimed to identify and validate miR-210 and miR-152 as non-invasive circulating biomarkers, for the diagnosis and staging of BC patients, confirming their involvement in tumor angiogenesis. RT-qPCR was performed and MiRNA expression analysis was obtained from plasma and fragments of BC and benign breast condition (BBC) women patients, plus healthy subjects. Additionally, the immunohistochemistry technique was carried out to analyze the expression of target proteins. Tumor fragments showed increased expression of oncomiR-210 and decreased expression of miR-152 tumoral suppressor. Both miRNAs were increased in plasma samples from BC pati

SUBMITTER: Lopes BC 

PROVIDER: S-EPMC7825421 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature