Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach.

ABSTRACT: The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8⁺ T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8⁺ T-cell epitopes allowed identifying a 19-mer peptide

SUBMITTER: Bosch-Camos L 

PROVIDER: S-EPMC7825812 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature