The Adaptive Potential of the Middle Domain of Yeast Hsp90.

ABSTRACT: The distribution of fitness effects (DFEs) of new mutations across different environments quantifies the potential for adaptation in a given environment and its cost in others. So far, results regarding the cost of adaptation across environments have been mixed, and most studies have sampled random mutations across different genes. Here, we quantify systematically how costs of adaptation vary along a large stretch of protein sequence by studying the distribution of fitness effects of the same ≈2,300 amino-acid changing mutations obtained from deep mutational scanning of 119 amino acids in the middle domain of the heat shock protein Hsp90 in five environments. This region is known to be important for client binding, stabilization of the Hsp90 dimer, stabilization of the N-terminal-Middle an

SUBMITTER: Cote-Hammarlof PA 

PROVIDER: S-EPMC7826181 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature