Customized optical mapping by CRISPR-Cas9 mediated DNA labeling with multiple sgRNAs.

ABSTRACT: Whole-genome mapping technologies have been developed as a complementary tool to provide scaffolds for genome assembly and structural variation analysis (1,2). We recently introduced a novel DNA labeling strategy based on a CRISPR-Cas9 genome editing system, which can target any 20bp sequences. The labeling strategy is specifically useful in targeting repetitive sequences, and sequences not accessible to other labeling methods. In this report, we present customized mapping strategies that extend the applications of CRISPR-Cas9 DNA labeling. We first design a CRISPR-Cas9 labeling strategy to interrogate and differentiate the single allele differences in NGG protospacer adjacent motifs (PAM sequence). Combined with sequence motif labeling, we can pinpoint the single-base differences in highl

SUBMITTER: Abid HZ 

PROVIDER: S-EPMC7826249 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature