A pathway for error-free non-homologous end joining of resected meiotic double-strand breaks.

ABSTRACT: Programmed DNA double-strand breaks (DSBs) made during meiosis are repaired by recombination with the homologous chromosome to generate, at selected sites, reciprocal crossovers that are critical for the proper separation of homologs in the first meiotic division. Backup repair processes can compensate when the normal meiotic recombination processes are non-functional. We describe a novel backup repair mechanism that occurs when the homologous chromosome is not available in Drosophila melanogaster meiosis. In the presence of a previously described mutation (Mcm5A7) that disrupts chromosome pairing, DSB repair is initiated by homologous recombination but is completed by non-homologous end joining (NHEJ). Remarkably, this process yields precise repair products. Our results provide support fo

SUBMITTER: Hatkevich T 

PROVIDER: S-EPMC7826270 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature