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The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.

ABSTRACT: At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should, therefore, be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers.

SUBMITTER: Liu J

PROVIDER: S-EPMC7826384 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.

Liu Jie J Jang Ji Yong JY Pirooznia Mehdi M Liu Shihui S Finkel Toren T

Proceedings of the National Academy of Sciences of the United States of America 20210101 3

At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion as well as how this model allows for the characterization of muscle-derived serum proteins that either increas ...[more]

PMID: 33431665

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