Candidate Effectors from Botryosphaeria dothidea Suppress Plant Immunity and Contribute to Virulence.

ABSTRACT: Fungal effectors play important roles in host-pathogen interactions. Botryosphaeria dothidea is an ascomycetous fungus that is responsible for the diseases of hundreds of woody plant species, including apple ring rot, which seriously affects apples worldwide. However, little is known about the effectors of B. dothidea. In this study, we analyzed the B. dothidea genome and predicted 320 candidate effector genes, 124 of which were successfully amplified and cloned. We investigated the effects of these genes on plant cell death in Nicotiana benthamiana while using a transient expression system. Twenty-four hours after initial inoculation with Agrobacterium tumefaciens cells carrying candidate effectors, the infiltrated leaves were challenged with A. tumef

SUBMITTER: Zhang CJ 

PROVIDER: S-EPMC7826910 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature