Genetic Manipulation of the Brassicaceae Smut Fungus Thecaphora thlaspeos.

ABSTRACT: Investigation of plant-microbe interactions greatly benefit from genetically tractable partners to address, molecularly, the virulence and defense mechanisms. The smut fungus Ustilago maydis is a model pathogen in that sense: efficient homologous recombination and a small genome allow targeted modification. On the host side, maize is limiting with regard to rapid genetic alterations. By contrast, the model plant Arabidopsis thaliana is an excellent model with a vast amount of information and techniques as well as genetic resources. Here, we present a transformation protocol for the Brassicaceae smut fungus Thecaphora thlaspeos. Using the well-established methodology of protoplast transformation, we generated the first reporter strains expressing fluorescent proteins to follow mating. As a

SUBMITTER: Plucker L 

PROVIDER: S-EPMC7826943 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature