Promoterless Transposon Mutagenesis Drives Solid Cancers via Tumor Suppressor Inactivation.

ABSTRACT: A central challenge in cancer genomics is the systematic identification of single and cooperating tumor suppressor gene mutations driving cellular transformation and tumor progression in the absence of oncogenic driver mutation(s). Multiple in vitro and in vivo gene inactivation screens have enhanced our understanding of the tumor suppressor gene landscape in various cancers. However, these studies are limited to single or combination gene effects, specific organs, or require sensitizing mutations. In this study, we developed and utilized a Sleeping Beauty transposon mutagenesis system that functions only as a gene trap to exclusively inactivate tumor suppressor genes. Using whole body transposon mobilization in wild type mice, we observed that cumulative gene inactivation can drive

SUBMITTER: Aiderus A 

PROVIDER: S-EPMC7827284 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature