Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation.

ABSTRACT: Dopaminergic (DA) neurons derived from human pluripotent stem cells (hPSCs) represent a renewable and available source of cells useful for understanding development, developing disease models, and stem-cell therapies for Parkinson's disease (PD). To assess the utility of stem cell cultures as an in vitro model system of human DA neurogenesis, we performed high-throughput transcriptional profiling of ~20,000 ventral midbrain (VM)-patterned stem cells at different stages of maturation using droplet-based single-cell RNA sequencing (scRNAseq). Using this dataset, we defined the cellular composition of human VM cultures at different timepoints and found high purity DA progenitor formation at an early stage of differentiation. DA neurons sharing similar molecular identities to those found in au

SUBMITTER: Nilsson F 

PROVIDER: S-EPMC7827700 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature