MALDI TOF Mass Spectrometry Imaging of Blood Smear: Method Development and Evaluation.

ABSTRACT: The aim of this study was to develop and evaluate matrix assisted LASER desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry imaging (MSI) of blood smear. Integrated light microscope and MALDI IT-TOF mass spectrometer, together with a matrix sublimation device, were used for analysis of blood smears coming from healthy male donors. Different blood plasma removal, matrix deposition, and instrumental settings were evaluated using the negative and positive ionization modes while agreement between the light microscopy images and the lateral distributions of cellular marker compounds served as the MSI quality indicator. Red and white blood cells chemical composition was analyzed using the differential m/z expression. Five seconds of exposure to ethanol followed by the 5 min of 9

SUBMITTER: Debeljak Z 

PROVIDER: S-EPMC7827909 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature