Novel Regeneration Approach for Creating Reusable FO-SPR Probes with NTA Surface Chemistry.

ABSTRACT: To date, surface plasmon resonance (SPR) biosensors have been exploited in numerous different contexts while continuously pushing boundaries in terms of improved sensitivity, specificity, portability and reusability. The latter has attracted attention as a viable alternative to disposable biosensors, also offering prospects for rapid screening of biomolecules or biomolecular interactions. In this context here, we developed an approach to successfully regenerate a fiber-optic (FO)-SPR surface when utilizing cobalt (II)-nitrilotriacetic acid (NTA) surface chemistry. To achieve this, we tested multiple regeneration conditions that can disrupt the NTA chelate on a surface fully saturated with His6-tagged antibody fragments (scFv-33H1F7) over ten regeneration cycles. The best surface regenerati

SUBMITTER: Qu JH 

PROVIDER: S-EPMC7828519 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature