Targeting TRPV1-mediated autophagy attenuates nitrogen mustard-induced dermal toxicity.

ABSTRACT: Nitrogen mustard (NM) causes severe vesicating skin injury, which lacks effective targeted therapies. The major limitation is that the specific mechanism of NM-induced skin injury is not well understood. Recently, autophagy has been found to play important roles in physical and chemical exposure-caused cutaneous injuries. However, whether autophagy contributes to NM-induced dermal toxicity is unclear. Herein, we initially confirmed that NM dose-dependently caused cell death and induced autophagy in keratinocytes. Suppression of autophagy by 3-methyladenine, chloroquine, and bafilomycin A1 or ATG5 siRNA attenuated NM-induced keratinocyte cell death. Furthermore, NM increased transient receptor potential vanilloid 1 (TRPV1) expression, intracellular Ca²⁺ content, and the activitie

SUBMITTER: Chen M 

PROVIDER: S-EPMC7829253 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature