Assessing the Resident Progenitor Cell Population and the Vascularity of the Adult Human Meniscus.

ABSTRACT:

Purpose

To identify, characterize, and compare the resident progenitor cell populations within the red-red, red-white, and white-white (WW) zones of freshly harvested human cadaver menisci and to characterize the vascularity of human menisci using immunofluorescence and 3-dimensional (3D) imaging.

Methods

Fresh adult human menisci were harvested from healthy donors. Menisci were enzymatically digested, mononuclear cells isolated, and characterized using flow cytometry with antibodies against mesenchymal stem cell surface markers (CD105, CD90, CD44, and CD29). Cells were expanded in culture, characterized, and compared with bone marrow-derived mesenchymal stem cells. Trilineage differentiation potential of cultured cells was determined. Vasculature of menisci was mapped in 3D

SUBMITTER: Chahla J 

PROVIDER: S-EPMC7829352 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature