CopR, a Global Regulator of Transcription to Maintain Copper Homeostasis in Pyrococcus furiosus.

ABSTRACT: Although copper is in many cases an essential micronutrient for cellular life, higher concentrations are toxic. Therefore, all living cells have developed strategies to maintain copper homeostasis. In this manuscript, we have analyzed the transcriptome-wide response of Pyrococcus furiosus to increased copper concentrations and described the essential role of the putative copper-sensing metalloregulator CopR in the detoxification process. To this end, we employed biochemical and biophysical methods to characterize the role of CopR. Additionally, a copR knockout strain revealed an amplified sensitivity in comparison to the parental strain towards increased copper levels, which designates an essential role of CopR for copper homeostasis. To learn more about the CopR-regulated ge

SUBMITTER: Grunberger F 

PROVIDER: S-EPMC7830388 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature