Eicosanoid Content in Fetal Calf Serum Accounts for Reproducibility Challenges in Cell Culture.

ABSTRACT: Reproducibility issues regarding in vitro cell culture experiments are related to genetic fluctuations and batch-wise variations of biological materials such as fetal calf serum (FCS). Genome sequencing may control the former, while the latter may remain unrecognized. Using a U937 macrophage model for cell differentiation and inflammation, we investigated whether the formation of effector molecules was dependent on the FCS batch used for cultivation. High resolution mass spectrometry (HRMS) was used to identify FCS constituents and to explore their effects on cultured cells evaluating secreted cytokines, eicosanoids, and other inflammatory mediators. Remarkably, the FCS eicosanoid composition showed more batch-dependent variations than the protein composition. Efficient uptake of fatty aci

SUBMITTER: Niederstaetter L 

PROVIDER: S-EPMC7830683 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature