Dynamic effects of calcium on in vivo and ex vivo platelet behavior after trauma.

ABSTRACT:

Background

Mobilization of intra and extracellular calcium is required for platelet activation, aggregation, and degranulation. However, the importance of alterations in the calcium-platelet axis after injury is unknown. We hypothesized that in injured patients, in vivo initial calcium concentrations (pretransfusion) predict ex vivo platelet activation and aggregation, viscoelastic clot strength, and transfusion of blood products. We additionally hypothesized that increasing calcium concentrations ex vivo increases the expression of platelet activation surface receptors and platelet aggregation responses to agonist stimulation in healthy donor blood.

Methods

Blood samples were collected from 538 trauma patients on arrival to the emergency department. Standard assays (includi

SUBMITTER: Matthay ZA 

PROVIDER: S-EPMC7830742 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature