Global transcriptome changes of elongating internode of sugarcane in response to mepiquat chloride.

ABSTRACT:

Background

Mepiquat chloride (DPC) is a chemical that is extensively used to control internode growth and create compact canopies in cultured plants. Previous studies have suggested that DPC could also inhibit gibberellin biosynthesis in sugarcane. Unfortunately, the molecular mechanism underlying the suppressive effects of DPC on plant growth is still largely unknown.

Results

In the present study, we first obtained high-quality long transcripts from the internodes of sugarcane using the PacBio Sequel System. A total of 72,671 isoforms, with N50 at 3073, were generated. These long isoforms were used as a reference for the subsequent RNA-seq. Afterwards, short reads generated from the Illumina HiSeq 4000 platform were used to compare the differentially expressed genes in both

SUBMITTER: Chen R 

PROVIDER: S-EPMC7831198 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature