Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples.

ABSTRACT: The pandemic caused by SARS-CoV-2 has triggered an extraordinary collapse of healthcare systems and hundred thousand of deaths worldwide. Following the declaration of the outbreak as a Public Health Emergency of International Concern by the World Health Organization (WHO) on January 30th, 2020, it has become imperative to develop diagnostic tools to reliably detect the virus in infected patients. Several methods based on real time reverse transcription polymerase chain reaction (RT-qPCR) for the detection of SARS-CoV-2 genomic RNA have been developed. In addition, these methods have been recommended by the WHO for laboratory diagnosis. Since most of these protocols are based on the use of fluorogenic probes and one-step reagents (cDNA synthesis followed by PCR amplification in the same tub

SUBMITTER: Pereira-Gomez M 

PROVIDER: S-EPMC7831559 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature