Novel mouse model recapitulates genome and transcriptome alterations in human colorectal carcinomas.

ABSTRACT: Human colorectal carcinomas are defined by a nonrandom distribution of genomic imbalances that are characteristic for this disease. Often, these imbalances affect entire chromosomes. Understanding the role of these aneuploidies for carcinogenesis is of utmost importance. Currently, established transgenic mice do not recapitulate the pathognonomic genome aberration profile of human colorectal carcinomas. We have developed a novel model based on the spontaneous transformation of murine colon epithelial cells. During this process, cells progress through stages of pre-immortalization, immortalization and, finally, transformation, and result in tumors when injected into immunocompromised mice. We analyzed our model for genome and transcriptome alterations using ArrayCGH, spectral karyotyping (S

SUBMITTER: McNeil NE 

PROVIDER: S-EPMC7832084 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature